Publications ( Cited by SCI )

COMPLETE SEPARATION OF ALBUMIN AND HEMOGLOBIN BY METAL CHELATE AFFINITY-CHROMATOGRAPHY




作者: YANG BL, GOTO S
发表/完成日期: 1991-06-12
期刊名称: SEPARATION SCIENCE AND TECHNOLOGY
期卷: 26 (5)
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论文简介
Hemoglobin was adsorbed on Zn2+ chelating Sepharose 6B but albumin was not adsorbed. Then albumin and hemoglobin could be separated completely by metal chelate affinity chromatography. In a conventional columnwise adsorption step, unusual breakthrough curves were observed because some parts of metal ions could be released from the adsorbent. A combined process with batchwise adsorption and columnwise elution was used to avoid this problem and to shorten the operating time.
IDS Number: FW828