Publications ( Cited by SCI )

Hydrolysis of O-nitrophenyl-beta-D-galactoside by an enzyme immobilized on perfluorocarbon in a tapered column reactor




作者: Yang BL, Shinoda N, Goto S
发表/完成日期: 1998-03-15
期刊名称: COLLOIDS AND SURFACES B-BIOINTERFACES
期卷: 10 (3)
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论文简介
An enzyme, beta-galactosidase, was immobilized on the surface of perfluorocarbon (PFC) droplets using perfluorobenzyl chloride as a modifier. The optimal conditions for introducing the enzyme to the PFC were determined in order to find the maximal activity for the hydrolysis of O-nitrophenyl-beta-D-galactoside. This novel immobilized enzyme was also successfully used in a tapered column reactor. Experimental results indicated that PFC droplets tended to stabilize the operation in a tapered column reactor with upflow and allowed a much wider range of operating conditions than a conventional cylindrical column reactor. (C) 1998 Elsevier Science B.V.
IDS Number: ZD192