Publications ( Cited by SCI )

Investigation on Hydrogen Peroxide Biosensor Based on Myoglobin/Porous Carbon Nanofiber/Room Temperature Ionic Liquid Composite Film

作者: Liu B (Liu, Bin)1,2, Yang BL (Yang, Bo-Lun)1
发表/完成日期: 2009-12-22
期卷: 7( 6 ):

A novel biosensor was fabricated by immobilizing myoglobin (Mb) into a novel nanocomposite (PCNFs/BMIM . PF6) consisted of two unique materials (PCNFs and BMIM . PF6), and the direct electron transfer of Mb and electrocatalytic ability of the biosensor to the reduction of hydrogen peroxide (H2O2) was also investigated. The Mb/PCNF/RTIL film was also characterized by UV-visible spectroscopy and the result showed that Mb in the composite matrix could retain its native structure A pair of well-defined, quasi-reversible cyclic voltammetric peaks at -0.284 V (vs. SCE) in pH 7 0 buffers, characteristic peaks of the protein heme Fe(III)/Fe(II) redox couples, were observed, suggesting that the composite matrix of PCNF/RTIL facilitated the direct electron transfer of Mb and provided a suitable microenvironment for Mb to retain its bioactivity Moreover, the resulting biosensor also exhibited an excellent electrocatalytic activity to the reduction of H2O2 with a detection limit of 4 mu M (S/N = 3). The apparent Michaelis-Menten constant (K-m(aPP)) was estimated to be 0.056 mM The study proved that the combination of RTIL with PCNFs is able to open up new opportunities for the design of enzymatic biosensors; with potential applications in practice