论文期刊

Sculpting nanoparticle dynamics for singlebacteria-level screening and direct bindingefficiency measurement




作者: Y.Z. Shi, S. Xiong, ...,J.H. Wu, T.N. Chen, ,,,,& A.Q. Liu
发表/完成日期: 2018-02-28
期刊名称: NATURE COMMUNICATIONS
期卷: Vol.9
相关文章: NATURE COMMUNICATIONS.pdf   
论文简介
Particle trapping and binding in optical potential wells provide a versatile platform for various
biomedical applications. However, implementation systems to study multi-particle contact
interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we
demonstrate the precise control of particle interactions and functions such as controlling
aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the
mechanical interactions among aggregated particles. The optofluidic lattice also enables
single-bacteria-level screening of biological binding agents such as antibodies through
particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined
directly, selectively, quantitatively and efficiently. This work enriches the fundamental
mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.