研究方向：纳米抗体与蛋白质工程

师从纳米抗体创始人Serge Muyldermans教授，以纳米抗体、结构生物学、分子影像学为技术手段，以实验性炎症和肿瘤动物模型为平台，利用荧光和核素，对免疫细胞和肿瘤细胞进行示踪和靶向治疗，以及博士后期间，在Robert Campbell教授课题组进行荧光探针开发及光遗传学方面的研究。

重点研发青年科学家项目骨干1项，国家自然科学基金2项，教育部留学基金1项，教育部重点实验室青年骨干项目2项，陕西省自然科学基金等。

在Nucleic acids research (IF:19.1)，Journal of Nuclear Medicine(IF：11.8)、Nature Communications (IF:17.6, F1000，ESI 1% ) 等国际知名杂志上发表论文20余篇。单篇高被引470次，获得单域重链抗体相关欧洲专利1项，获得中国发明专利5项，申请中国发明专利1项。

担任省一流课程《生物化学》负责人，负责全国高等教材双语教材1部《医学分子生物学》人卫版，获批教改课题7项，参与教改项目14项，发表教改论文5篇。获得“23年西安交通大学优秀班主任”、“23年陕西省青年教师比赛 三等奖”“23年全国来华留学生本科教育青年教师英语授课教学能手”，担任“侯宋濂基础医学拔尖班2.0工作组”核心成员。

世界分子影像学会会员（WMIS），欧洲分子影像学会会员（ESMI）、中国生物化学与分子生物学会员、中国医药教育协会常务委员，中国微米纳米技术学会会员，陕西省医学会会员。与比利时布鲁塞尔自由大学，美国西北大学，加拿大阿尔伯塔大学以及日本东京大学，三亚崖州湾科技城建立稳定合作。

Research Interests:

Nanobody/complement receptor/cancer immunology/in vivo imaging: Dr. Zheng has been working with Nanobody in Vrije Universiteit Brussel and Vlaams Instituut voor Biotechnologic (Belgium) during Ph.D. since the great potential of the smallest antibody in preclinical and clinical research. Dr. Zheng previous research was focused on the in vivo monitoring of arthritic and hepatitis mouse models using complement receptor targeting nanobody as molecular imaging SPECT/CT prober. Her present research topics include cancer-associated macrophage in vivo imaging, generating nanobody targeting immune checkpoint molecular used for immunotherapy. In addition to cancer immunology, She has also worked on a collaborative project in protein structure and function studies of Nanobody recognition molecular mechanism. Recently, Dr. Zheng collaborated with Dr. Robert Campbell at the University of Alberta in Canada, she has added a new aspect to work on developing a Genetically encoded Fluorescence biosensor using Nanobody. Dr. Zheng published in top journals such as Nucleic Acids Research, Nature Communications, Scientific Reports, and Journal of Nuclear Medicine, etc.

人才项目:

1. 获得2018年医学部第二批国家级人才后备人选支持计划-攀登人才计划

2. 获得陕西省高校科协青年人才托举计划支持 

个人获奖:

1.2023年全国来华留学生本科教育青年英语授课 教学能手（二等奖）

2.2023年秦创原 陕西省科技工作者创新创业大赛 三等奖

3.2023年西安交通大学 优秀班主任

4.2023年陕西省青年教师教学大赛，三等奖

5.2022年陕西省科技工作者创新创业大赛 三等奖

6.2022年第三节全国健康长寿奖，三等奖

7.2021年第二届全国健康长寿奖，银奖，项目获得创业资助立项

8.“单域重链抗体在精准生物诊疗一体化中的应用”陕西省科技工作者创新创业大赛，2017，铜奖

9.  The 5th Hsien Wu and Ray Wu Symposia，2017，青年论坛奖 

10.  2016年全国生物化学与分子生物学年会，2016，最佳壁报奖

指导学生获奖：

1.首届教育部基础医学“101计划”拔尖学生创新论坛 （闫锦、谢宇、于洋、刘昱安） 三等奖

2.西安交通大学“腾飞杯”创业竞赛 2022年，三等奖（指导老师）

  3.  莫缓椰，刘彤，张妮，Precise 单域抗体影像学，2018微软“创新杯”陕西高校大学生创新创业大赛，优秀奖 （指导老师）

  4.  莫缓椰，刘彤，张妮，李子乾，杜宇芩，费宇，单域重链抗体在肿瘤精准诊断中的应用，2018年 西安交通大学大学生创新创业项目，三等奖（指导老师）

  5. 莫缓椰，刘彤，张妮，李子乾，杜宇芩，费宇，西安交通大学第十二届“腾飞杯”创业竞赛，三等奖（指导老师）

专利及科研转化：

（1）授权欧洲专利1项，中国发明专利6项，申请中国发明专利1项，申请国际PCT发明专利1项

（3）“一种Vsig4纳米抗体及其抗原表位鉴定方法和应用”和“一种Clec4F纳米抗体的应用”，两个专利实现专利转化，获得陕西省秦创原春种种子基金100万元资助。参与2022年参与“纳米抗体技术”成果转化框架协议（横向项目）。

Publications：

(1) Wenbo He ^#, Ke Wu ^#, Zhenlin Ouyang, Yixin Bai, Wen Luo, Di Wu, Hao An, Yucheng Guo, Min Jiao, Qian Qin, Jiaxin Zhang, Yi Wu, Junjun She, Peter M. Hwang, Fang Zheng, Li Zhu*, Yurong Wen*. (2023) Structure and Assembly of Type VI secretion system cargo delivery vehicle. Cell Reports. 42. 112781. (Cell 子刊, IF: 9.995)

(2) Min Jiao, Wenbo He, Zhenlin Ouyang, Qian Qin, Yucheng Guo, Jiaxin Zhang, Yixin Bai, Xiaolong Guo, Qinyue Yu, Junjun She, Peter M. Hwang, Fang Zheng, Yurong Wen*. (2023) Mechanistic and Structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure. 31. 935-947. (Cell 子刊)

(3) Yucheng Guo, Zhenlin Ouyang, Wenbo He, Jiaxin Zhang, Qian Qin, Min Jiao, Serge Muyldermans, Fang Zheng*, Yurong Wen*. Screening and epitope characterization of diagnostic nanobody against total and activated Bacteroides fragilis toxin. Front Immunol. 2023 Feb 10;14:1065274.  doi: 10.3389/fimmu.2023.1065274.  eCollection 2023.

(4) Qin Q, Liu H, He W, Guo Y, Zhang J, She J, Zheng F, Zhang S, Muyldermans S, Wen Y*. Single Domain Antibody application in bacterial infection diagnosis and neutralization.Front Immunol. 2022 Sep 29;13:1014377. doi: 10.3389/fimmu.2022.1014377. eCollection 2022. PMID: 36248787

(5) Zheng F; Pang, Y; Li L; Pang Y; Zhang J; Wang X; Raes G. Frontiers in Immunology. 2022，13：978513。（ IF: 8.7，第一作者，综述）

(6) Zhenlin Ouyang#, Fang Zheng#, Li Zhu#, Jan Felix, Di Wu, Ke Wu, Irina Gutsche, Yi Wu, Peter M Hwang, Junjun She*, Yurong Wen*. Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience. 2021,24(5): 102476. DOI: https://doi.org/10.1016/j.isci.2021.102476 (Cell子刊, IF: 5.35, 并列第一作者) PMID: 34113820

(7) Fang Zheng, Jinhong Zhou, Zhenlin Ouyang, Jiaxin Zhang, Xinyi Wang, Serge Muyldermans, Jo Van Ginderachter, Nick Devoogdt, Yurong Wen*, Steve Schoonooghe* and Geert Raes*.Development and characterization of nanobodies targeting the Kupffer cell. Frontiers in Immunology, 2021, 12 : 641819. DOI10.3389/fimmu.2021.641819(IF:7.56, 第一作者 ) PMID: 33692811

(8) Zhenlin Ouyang#, Fang Zheng#, Yingmei Pei, Jinhong Zhou, Keqing Wen, Miao Han, Peter M. Hwang, M. Joanne Lemieux, Yurong Wen*. (2019) Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR. Nucleic Acids Research. 2019, 47(21):11418-11429.（IF: 16.97,并列第一作者）DOI10.1093/nar/gkz891 PMID: 31598698

(9) Fang Zheng, Siyu Luo , Zhenlin Ouyang, Jinhong Zhou, Huanye Mo, Steve Schoonoogh, Serge Muyldermans , Patrick De Baetselier, Geert Raes *,and Yurong Wen*. NIRF-Molecular Imaging with Synovial Macrophages-Targeting Vsig4 Nanobody for Disease Monitoring in a Mouse Model of Arthritis. International Journal of Molecular sciences. July 2019.20（13） （IF: 5.923, 第一作者）PMID: 31288389 PMCID: PMC6651725 DOI: 10.3390/ijms20133347

(10) Zhenlin Ouyang, Jan Felix, Jinhong Zhou, Yingmei Pei, Bohan Ma, Peter M. Hwang  M. Joanne Lemieux, Irina Gutsche, Fang Zheng^#  Yurong Wen#. Trimeric structure of the mouse Kupffer cell C‐type lectin receptor Clec4f. FEBS letters, Augest 2019.541（1）：189-198 （SCI IF：4.12 通讯作者）DOI10.1002/1873-3468.13565 PMID: 31369681

(11) Yurong Wen^#*, Zhenlin Ouyang^#, Yue Yu, Xiaorong Zhou, Yingmei Pei, Bart Devreese, Paul G. Higgins, Fang Zheng*. Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region. Nucleic Acids Research. SEP 2017,45（16）：9773-9787）（SCI IF：16.97 通讯作者）PMID: 28934482 PMCID: PMC5766154 DOI: 10.1093/nar/gkx624

(12) Wen Y^#*, Ouyang Z^#, Devreese B, He W, Shao Y, Lu W, Zheng F*.Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain. Protein Sci. 2017 Jul 30. 26(10)：2073-2082 （通讯作者 SCI IF：6.72）PMID: 28758290 PMCID: PMC5606546 DOI: 10.1002/pro.3245

(13) Yurong Wen#, Zhenlin Ouyang#, Steve Schoonooghe, Siyu Luo, Patrick De Baetselier, Wuyuan Lu, Serge Muyldermans, Geert Raes, Fang Zheng*. (2017). Structural evaluation of a nanobody targeting complement receptor Vsig4 and its cross reactivity. Immunobiology. 222(6): 807-813. (IF: 3.14, 通讯作者) PMID: 27889311 DOI: 10.1016/j.imbio.2016.11.008

(14) Zheng Fang#, Sparkes Amanda#, De Baetselier Patrick, Schoonooghe, Steve, Stijlemans Benoit, Muyldermans, Serge, Flamand Veronique, Van Ginderachter Jo A, Devoogdt Nick, Raes Geert, Beschin Alain*. Molecular Imaging with Kupffer Cell-Targeting Nanobodies for Diagnosis and Prognosis in Mouse Models of Liver Pathogenesis. MOLECULAR IMAGING AND BIOLOGY. Feb 2017, 19 (1):49-58.((IF: 3.48, 第一作者) )PMID: 27339464 DOI: 10.1007/s11307-016-0976-3

(15) Zheng F, Perlman H, Matthys P, Wen Y, Lahoutte T, Muyldermans S, Lu S, De Baetselier P, Schoonooghe S, Devoogdt N, Raes G. Specificity Evaluation and Disease Monitoring in Arthritis Imaging with Complement Receptor of the Ig superfamily targeting Nanobodies. Scientific Reports. 2016 Oct 25;6:35966. doi: 10.1038/srep35966. （SCI IF：5.2）

(16) Charlotte L. Scott, Fang Zheng, Patrick De Baetselier, Liesbet Martens, Yvan Saeys, Sofie De Prijck, Saskia Lippens, Chloé Abels, Steve Schoonooghe, Geert Raes, Nick Devoogdt, Bart N. Lambrecht, Alain Beschin* and Martin Guilliams*. Bone marrow derived monocytes give rise to self-renewing and fully differentiated Kupffer cells. Nature communications. 2016 Jan. （SCI IF：12.5，Nature子刊，SCI 高被引论文, 被引346）

(17) Fang Zheng^#, Amanda Sparkes^#, Patrick De Baetselier, Steve Schoonooghe, Benoit Stijlemans, Serge Muyldermans,Véronique Flamand, Jo A Van Ginderachter, Nick Devoogdt, Geert Raes, Alain Beschin. Molecular Imaging with Kupffer Cell-Targeting Nanobodies for Diagnosis and Prognosis in Mouse Models of Liver Pathogenesis. Mol Imaging Biol (2016). DOI: 10.1007/s11307-016-0976-3  （SCI IF：3.1）

(18) Yurong Wen^#, Zhenlin Ouyang^#, Steve Schoonooghe, Siyu Luo4, Patrick De Baetselier, Wuyuan Lu, Serge Muyldermans, Geert Raes*, Fang Zheng*. Structural evaluation of a Nanobody targeting complement receptor Vsig4 and its cross reactivity. Immunobiology, 2016 . （SCI IF：3.4）

(19) Zheng F, Put S, Bouwens L, Lahoutte T, Matthys P, Muyldermans S, De Baetselier P, Devoogdt N, Raes G, Schoonooghe S*. Molecular imaging with macrophage CRIg-targeting Nanobodies for early and preclinical diagnosis in a mouse model of rheumatoid arthritis. Journal of Nuclear Medicine.  2014 May;55(5):824-9. （SCI IF：10.5，影像学领域排名前三期刊）

(20) Zheng F, Devoogdt N, Amanda Sparkes, Chlotthys P, Muyldermans S, De Baetselier P, Devoogdt N, Schoonooghe S, Raes G*. Monitoring resident liver macrophages using Nanobodies targeting Vsig4 : Concanavalin A induced acute hepatitis as paradigm. Immunobiology, 2015 Feb;220(2):200-9. （SCI IF：3.4）

(21)郑芳; 罗思羽; 韩燕; 宁启兰; 温玉荣*，靶向巨噬细胞膜蛋白Vsig4特异性纳米抗体的构建和筛选，西安交通大学学报(医学版), 2017.1, 38(1): 7~12，核心期刊

(22)郑芳; 罗思羽; 韩燕; 宁启兰; 温玉荣*，靶向巨噬细胞膜蛋白Vsig4特异性纳米抗体的构建和筛选，西安交通大学学报(医学版), 2017.1, 38(1): 7~12，核心期刊

(23) Fang ZHENG; Yurong WEN(*)，The Necessity of Experimental Animal Training Program in Chinese Institution and University， 2016 International Conference on Education, Science and Education Management (ESEM 2016).