王嗣岑发表论文

Screening active anti-breast cancer compounds from cortex magnolia officinalis by MCF-7 cell membrane chromatography coupled with UHPLC-ESI-MS/MS




作者: Xiaoshuang He, Ping Zhang, Muhammad Saqib, Xiaofang Hou*,  Sicen Wang*
发表/完成日期: 2017-07-18
期刊名称: Anal Meth
期卷: 2017,9
相关文章: 贺晓双 ana meth.pdf   
论文简介
Breast cancer is one of the most frequent malignant tumors in females. Extracts from Cortex Magnolia
Officinalis (CMO) have shown the ability to inhibit the growth of breast cancer cells. In the present study,
we developed MCF-7 cell membrane chromatography (CMC) combined with a UHPLC-ESI-MS/MS
method to screen and identify the active components from the extract of CMO. Sorafenib and
dexamethasone acetate were used as positive and negative control drugs, respectively, to investigate the
suitability of this system. Under optimum experimental conditions, two components showed strong
retention characteristics on the MCF-7 cell membrane chromatographic column. These compounds
were identified as magnolol and honokiol by UHPLC-ESI-MS/MS. Cytotoxicity assay and Hoechst 33258
staining were done to investigate the anti-proliferative activity of the two compounds. The MTT results
showed that MCF-7 cell viability decreased gradually in a dose-dependent manner during 48 h. The IC50
of magnolol and honokiol were 85.4 mM and 58.1 mM, respectively. After Hoechst 33258 staining, the
cells were observed with bright blue nuclei due to karyopyknosis and chromatin condensation. The
screening results showed that both magnolol and honokiol were promising active components of CMO
extract having an inhibitory effect on MCF-7 cell growth. This two-dimensional method CMC-UHPLCESI-
MS/MS could have potential utility for screening target components from traditional Chinese
medicines.