| 论文简介 |
Stimulated response molecularly imprinted polymers (MIPs) have attracted wide attention not only because of their intriguing response to external stimuli, but also due to the desired molecular recognition ability. Herein, a novel dual responsive proteins MIPs with both photonic and magnetic stimuli has been developed by surface imprinting polymerization, where double bonds modified Fe3O4 nanoparticles, a water-soluble azobenzene containing 4-[(4-methacryloyloxy)phenylazo]benzenesulfonic acid and bovine hemoglobin (BHb) were used as magnetic core, photoswitchable functional monomer and template protein, respectively. The resultant MIPs possessed a typical spherical structure with a relative rough imprinted layer of 15nm thickness. Moreover, the MIPs had good binding behavior toward BHb with fast binding kinetics, high adsorption capacity and satisfactory selectivity. The MIPs were proved to be well photo-controlled toward the template protein BHb under the alternating irradiation of365and 440nm: 31.4% BHbwasreleasedfrom theMIPsat 365nm and67.2% BHb was bound into the MIPs at 440nm. As a result, the MIPs were successfully applied to specifically extract template protein from actual biological sample. As a consequence, combining photocontrolled release and uptake, rapidly magnetic separation and specific recognition, the resultant MIPs with photonic and magnetic dual responsive abilities show significant advantages in the enrichment of trace protein in biological samples. |
(创新港)
