论文期刊

Detection of gastric carcinoma-associated antigen MG7-Ag in human serausing surface plasmon resonance sensor




作者: Xiangyi Fang, Jun Tie, Yonghonh Xie, et al.
发表/完成日期: 2010-11-25
期刊名称: Cancer Epidemiology
期卷: 34
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论文简介
Background: MG7-Ag is a kind of gastric cancer-specific tumor-associated antigen and has been
investigated to serve as a marker of gastric cancer for early diagnosis. Methods: Surface plasmon
resonance (SPR) sensor was used for the detection of MG7-Ag in the sera of gastric cancer patients to
develop an innovative, simple and rapid assay method for early diagnosis. The specificmonoclonal MG7
antibodies were used as capture and detection receptors which were immobilized on the surface of SPR
sensor chips for MG7-Ag identification in the human sera. The measurements include 9 cases of gastric
cancer patients and 2 cases of healthy blood donors and a MKN45 cancer cell lysate solution sample for
positive control. Results: The binding of MG7-Ag onto the sensor surface was observed from SPR spectra.
The sera of most gastric cancer patients revealed much higher expression level of MG7-Ag than healthy
human sera did in SPR measurement. Conclusion: The initial results demonstrate that the SPR biosensor
has the potential for its application in the early diagnosis of gastric cancer. However, more tests need to
be done to confirm the detection limitation and the criterion for cancer risk evaluation in early diagnosis.