(1.)Basic Information

Zhongmin,Tian., Ph.D.
Professor
Department of Biomedical and Bioengineering
School of Life Science and Technology
Xi’an Jiao-tong University
28# Xian-ning road, Xi’an, Shaanxi, P.R.China, 710049
room: 202
School of Life Science and Technology
Xi’an Jiao-tong University  
 
M.B. biology, North-west University, xian, China, 1997
Ph.D. Biomedical and bioengineering, Xian Jiao-tong university 2001
Postdoctoral Fellow, Nephrology, Medical college of Wisconsin, USA, 2006
Visiting scholar, Physiology, Medical College of Wisconsin, USA ,2009
 
Research areas:
u       Renal Physiology
u       Proteomics and metabonomics
 
My laboratory's research interest is in understanding and interpreting the development of salt sensitive hypertension. With kidney injury and metabolic disorders as the hypertension context, our current work is focused on:
1.        Proteomic analysis on salt-sensitive Hypertension
Arterial blood pressure of many essential hypertensive patients, exhibits an increased sensitivity to dietary salt intake. However, Sodium such an environmental factor has received the greatest attention. Approximately one third of the essential hypertensive population is responsive to high salt diet. SS-rat is considered as a useful rat model to understand human salt-sensitive hypertension. A number of physiological processes have been shown to be potentially involved in the increased blood pressure. Particularly, abnormalitiesin the kidneys appear to play a critical role. Quantitative trait loci analyses have indicated that regions of several Chromosomes are associated with the increased blood pressure salt-sensitivity in the SS rat. Howerer, the cut way to understand the development of salt-sensitive hypertension is to generate various Congenic or Consomic rat strains by substituting a small part of the genome from salt-resistant rat into the SS-rat to understand the genetic background.
SS-13BN from SS-rat except Chromosome 13 replaced by the Brown Norway, has become a valuable control strain for the study of the SS-rat. This made SS-13BN genetically 98.05% identical to SS. However, the blood pressure salt-sensitivity is significantly reduced in SS-13BN. Several interesting physiological differences have been identified between SS-13BN and SS rats, including a reduced level of hypertension renal injuryand cardiac hypertrophy.
There are only 1.95% difference in genetic background, and the arterial blood pressure had reduced significantly, but we still didn’t know what gene located on chromosome 13 and involved in the salt hypertension. So the purpose of my research team was to investigate differential expressed protein using current 2D DIGE technology so that we can understand the differential gene located on Chromosome 13.
The most interested results of our current work are fumarase and Hnrnp K, which we fund by 2D-DIGE analysis. (1) Fumarase locates on chromosome 13. (2) different modification lead to PI shift. (3) The activity of fumarase was detected and there were 2.88 fold difference between SS-rat and SS-13BN. (4) The activity of fumarase is also influenced by the ions concentration in the reaction medium in vitro, and reduced with increasing the concentration of sodium chloride in buffer. (5) Fumarase cDNA sequence analysis indicated that there was only one base difference between SS-rat and SS-13BN. This lead to glutimic acid was replaced by lysine at site 481 in SS-rat. Of course, we still found other interested proteins such as Hnrnp K. These results had been published on Hypertension 2009,2008 and Genome Research 2008
2.        the role of renal cellular metabolism in hypertension
In the area of cellular intermediary metabolism, my laboratory has made a completely novel discovery about a possible role of renal medullary insufficiency of fumarase activity in hypertension (Hypertension 2008, 2009). This discovery helped us to open a new direction of hypertension research focusing on the heretofore largely unrecognized importance of cellular intermediary metabolism in hypertension.  The initial discovery has generated significant excitement in the field as evidenced by recommendation by Faculty 1000 Biology and invitations from colleagues to present this specific work at national conferences or research institutions.
The cellular intermediary metabolism in renal medulla and cortex had been separated and identified by LC-MSMS and GC-MS. The ongoing work is collecting all these metabolism intermediate compounds. Recent discoveries that we are exploring in depth include novel roles of abnormalities in cellular metabolism in the development of salt sensitive hypertension and kidney injury.
 
3.    Phosphoproteomic
Protein phosphorylation is a key reversible modification that is involved in many cellular processes such as metabolism and transcription. Unfortunately, the dynamic nature of phosphorylation and the availability of multiple phosphorylation sites on the same protein add significant complexity to the analysis of cell signaling pathways through the monitoring of phosphoproteins. The purpose of our research work was to investigate whether such a strategy, the phosphoprotein enriched by the Pro-Q® Diamond Enrichment Kit, quantification by a 2D DIGE system and identification by Pro-Q and MALDI-TOF-MS, could be used to produce reliable quantitative data of phosphoproteins that accurately reflects biological differences in a cell system.
 
Technical Resources of our team
·         Agilent 7890a/5975c GC-MS
·         Agilent 1100 capillary HPLC with ion trap mass spectrometry(LC-MSMS)
·         Agilent capillary electrophoresis apparatus with MSD
·         Applied Biosystems Voyager-DE PRO MALDI-TOF-MS
·         Tecan Infinite M200 Pro Reader
·         Cell culture
·         Two-Dimensional Polyacrylamide Gel Electrophoresis (2-D DIGE) .
·         Biochemical analysis.
Grants:
v      Chinese National Natural Science Foundation Grants No. 81270767(700,000 RMB)2013.2-2017.2
v      Chinese National Natural Science Foundation Grants No. 31071029(360,000 RMB)2011.2-2014.2
v      New Century Excellent Talents in University. Grants No. NCET-08-0434, 500,000 Yuan/RMB 2008.9-2011.9
v      Natural Science Foundation of Shaanxi Province. Grants No. 2005C259, 20,000 Yuan/RMB 2005.9-2008.9
v      Natural Science Foundation of Xi’an Jiao-tong University Grants No.xjj2004117, 12.000 Yuan/RMB
Professional memberships:
Ø       Member, Chinese Physiological Society, committee of Renal Physiology
Ø      Vice–Chief, Ultrasound Medical Engineering Bio-effect Committee in China.
 
Selected publication
 
[1] He X, Liu Y, Usa K, Tian Z, Cowley AW Jr, Liang M. Ultrastructure of mitochondria and the endoplasmic reticulum in renal tubules of Dahl salt-sensitive rats. Am J Physiol Renal Physiol. 2014, 15; 306(10):F1190-7.
[2] Le Wang, Entai Hou, Liqing He, Zhengjun Wang, Na Sun, Lan Chen, Pu Jia, Xiaohui Zheng, Mingyu Liang, Zhongmin Tian, Analysis of metabolites in plasma reveals distinct metabolic features between Dahl salt-sensitive rats and consomic SS.13BN rats. Biochemical and Biophysical Research Communications,2014, 18;450 (1):863-9.
[3] Alison J. Kriegel, Yi Fang, Yong Liu, Zhongmin Tian et al.MicroRNA-Target Pairs in Human Renal Epithelial Cells treated with Transforming Growth Factor β1: A Novel Role of miR-382 Nucleic Acids Research. 2010, 38(22): 8338-8347. IF:7.479
[4] Zhongmin Tian, Yong Liu, Kristie Usa, Domagoj Mladinov, Yi Fang, Xiaoqiang Ding, Andrew S. Greene, Allen W. Cowley, Jr. Mingyu Liang. Novel Role of Fumarate Metabolism in Dahl-Salt Sensitive Hypertension. Hypertension. 2009,54: 255-260. IF=7.194
[5] Zhongmin Tian, Andrew S. Greene, Jennifer Pietrusz, Isaac R. Matus, and Mingyu Liang MicroRNAtarget pairs in the rat kidney identified by microRNA microarray, proteomic, and bioinformatic analysis Genome Research. 2008.18:404-411 IF=11.224
[6] Zhongmin Tian, Andrew S. Greene, Kristie Usa, Isaac R. Matus, Jesse Bauwens, Jennifer L. Pietrusz, Allen W. Cowley, Jr. and Mingyu Liang. Renal Regional Proteomes in Young Dahl Salt-Sensitive Rats. Hypertension. 2008,51(4):899-904. IF=7.194
[7] Zhongmin Tian, Mingxi Wan, Mingzhu Lu, Xiaodong Wang. The alteration of protein profile of walker 256 carinosarcoma cells during the apoptotic process induced by ultrasound. Ultrasound in Medicine & Biology, 2005, 31 (1): 121-128. IF=2.221
[8]      Zhongmin Tian, Mingxi Wan, Supin Wang, Juqing Kang. Effects of ultrasound and excipients on the function and structure of trypsin. Ultrasonics Sonochemistry 2004, 11(6), 399-404. IF=2.105
[9]      Zhongmin Tian, Mingxi Wan, Bo Wang, Supin Wang, Xiaoming Wu and Yusong Ruan. Effects of ultrasound on the structure and function of tumor necrosis factor-alpha. Ultrasound in Medicine & Biology, 2003, 29 (9): 1331-1339.
[10] Zhongmin Tian, Mingxi Wan, Zenglu Wang, Bo Wang. The preparation of genistein and LC-MS/MS on line analysis. Drug Development Research, 2004, 66 (1): 6-12.
[11]      Zhongmin Tian, Bo. Wang, Changxin Song, Weiping Li and Fangling Qin. The response of Lupinus albus L. roots to the signal from phosphorus deficient substrate. Russia plant physiology, 2004, 51(3): 396-401.
[12] Yi Feng, Zhongmin Tian, Ming-Xi Wan. Bio-effects of low intensity ultrasound in vitro: apoptosis, alteration of protein profile and potential molecular mechanism. Journal of Ultrasound in Medicine. 2010 29: 963-974 
[13] Yi Feng, Zhongmin Tian, Ming-Xi Wan,Zhao-Bin Zheng. Low intensity ultrasound induced apoptosis in human gastric carcinoma cellsWorld J Gastroenterol.200814314873-4879
[14] Yi Feng, Zhongmin Tian, Ming-Xi Wan, Zhao-Bin Zheng. Protein profile of human hepatocarcinoma cell line SMMC-7721: Identification and functional analysis[J]. World J Gastroenterol, 2007, 13(18): 2608-2614
[15] Mingyu Liang, Yong Liu, Domagoj Mladinov, Allen W. Cowley, Jr., Hariprasad Trivedi, Yi Fang, Xialian Xu, Xiaoqiang Ding, Zhongmin Tian. MicroRNA: A New Frontier in Kidney and Blood Pressure Research. Am J Physiol Renal Physiol, 2009American Journal of Physiology-renal physiology,
 
POSTER
[16]  Le Wang, Entai Hou, Mingyu Liang, Zhongmin Tian, Integrating GC/MS-based Metabolomics and Correlation Network Analyses Reveal Changes in Amino Acid Metabolism in Young Hypertensive Men High Blood Pressure Resarch 2014 14-HPBR-L-707-AHA
[17] Le Wang, Entai Hou, Zhengjun Wang, Na Sun, Liqing He, Lan Chen, Pu Jia, Xiaohui Zheng, Mingyu Liang, zhongmin Tian,Metabolomic Features in Dahl salt-sensitive rats and consomic SS.13BN rats High Blood Pressure Resarch 2014 14-HBPR-A-345-AHA
[18] Zhongmin Tian, Yong Liu, Kristie Usa, Domagoj Mladinov, Yi Fang, Xiaoqiang Ding, Andrew S. Greene, Allen W. Cowley, Jr. Mingyu Liang. A Novel Role of Fumarate Metabolism in Dahl-Salt Sensitive Hypertension. 63rd High Blood Pressure Research Conference, Hypertension. 2009, 54: E63-E64 
[19] Zhongmin Tian, Andrew S. Greene, Kristie Usa, Isaac R. Matus, Jennifer L. Pietrusz, Allen W. Cowley, Jr. and Mingyu Liang. Several dozen possible microRNA-target pairs in rat kidneys identified by microRNA microarray, proteomic, and bioinformatic analysis.61rd High Blood Pressure Research Conference, Hypertension, 2007. 50E120-120,
[20] Zhongmin Tian, Andrew S. Greene, Kristie Usa, Isaac R. Matus, Jennifer L. Pietrusz, Allen W. Cowley, Jr. and Mingyu Liang. Proteomic analysis of the renal medulla of Dahl salt-sensitive rats and consomic SS-13BN rats FASEB JOURNAL, 21(6):154-155, 2007
[21] Zhongmin Tian, Andrew S. Greene, Kristie Usa, Isaac R. Matus, Jennifer L. Pietrusz, Allen W. Cowley, Jr. and Mingyu Liang. Renal cortical and medullary proteomes in young Dahl salt-sensitive rats: Effects of chromosome 13 substitution and dietary salt intake. 61rd High Blood Pressure Research ConferenceHypertension. 2007. 50E120-120