A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67
发布时间：2025-04-30
点击次数：
- 发布时间：
- 2025-04-30
- 论文名称：
- A light-controlled switch after dual targeting of proliferating tumor cells via the membrane receptor EGFR and the nuclear protein Ki-67
- 发表刊物：
- Scientific Reports
- 摘要：
- Using nanotechnology for optical manipulation of molecular processes in cells with high spatial and
temporal precision promises new therapeutic options. Especially tumor therapy may proft as it requires a combination of both selectivity and an effective cell killing mechanism. Here we show a dual targeting approach for selective and efcient light-controlled killing of cells which are positive for epidermal growth factor receptor (EGFR) and Ki-67. Liposomes with the covalently linked EGFR antibody Erbitux enabled selective uptake of FITC-labeled Ki-67 antibody TuBB-9 in EGFR-positive cells pre-loaded with the photoactive dye BPD. After irradiation at 690 nm, BPD disrupted the endosomal membranes and delivered the antibodies to the nucleoli of the cells. The second irradiation at 490 nm activated the FITClabeled TuBB-9, which caused inactivation of the Ki-67 protein and subsequent cell death via apoptosis. Efcient cell killing was possible at nanomolar concentrations of TuBB-9 due to the effective transport by immune liposomes and the high efcacy of the Ki-67 light-inactivation. Delivery of the liposomal constructs and cell destruction correlated well with the EGFR expression pattern of different cell lines
(HeLa, OVCAR-5, MCF-7, and human fbroblasts), demonstrating an excellent selectivity.
- 合写作者：
- Sijia Wang, Gereon Hüttmann, Thomas Scholzen, Zhenxi Zhang,et al
- 卷号：
- 6
- 页面范围：
- 27032
- 是否译文：
- 否
- 发表时间：
- 2016-06-01