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论文期刊

论文标题    Specific Suppression of β-secretase Gene Expression by short interfering RNA in SK-N-SH Cells
作者    DONG Wei-jiang, HU Hai-tao, GONG Hui-lin
发表/完成日期    2006-03-08
期刊名称    Si Chuan Da Xue Xue Bao Yi Xue Ban
期卷    2006-37
相关文章   
论文简介    【Abstract】Objective: Double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various species. The current study was design to investigate whether short interfering RNAs(siRNAs) ofβ-site APP cleaving enzyme (BACE) can inhibit the expression of BACE in mammalian cells. Methods: To clone the gene of EGFP, U6 promoter andβ-secretase targeting siRNA by PCR, respectively. The PCR products were inserted into the retrovirus plasmid pLXSN. The interfering vector named pLXSN/EGFP-U6-siBACE. To produce the SK-N-SH cell line which can highly expressed BACE. Fluoroscopy and immunohistochemistry method to investigate the effect of BACE siRNA inhibits the BACE expression. Furthermore, to examine the production of Aβ. Results: The innterfering vector named pLXSN/EGFP-U6-siBACE was constructed successfully. The BACE siRNA can inhibit the expression of BACE in the SK-N-SH cell specifically and effectively，and reduced the production of Aβ.Conclusion: BACE siRNA inhibits the expression of BACE gene of mammalian, it provides an important experimental base for the application of RNA interference technology to prevention of AD.
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