论文期刊

论文标题    Adaptive spatial filtering based on region growing for automatic analysis in digital holographic microscopy
作者    Jiansu Li ; Zhao Wang ; Jianmin Gao ; Yun Liu and Junhui Huang
发表/完成日期    2015-03-10
期刊名称    Opt. Eng
期卷    54(3)
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论文简介    Abstract. The adaptive spatial filtering method is commonly adopted to extract the þ1 term spectrum in digital holography for real-time dynamic analysis. However, the typical filtering method is not satisfactory for automatic analysis, because the reset of the filtering window is needed to extract the area of the þ1 term spectrum. Therefore, an adaptive spatial filtering method based on region growing and the characteristic of the spectrum separation is proposed. Its filtering window is automatically formed by region growing. The key parameters, including threshold and seed point, are set by the intensity distribution of the hologram spectrum. Then the adaptive filtering extracting the þ1 term spectrum is realized by multiplying the hologram spectrum by the filtering window. Compared to the typical filtering method, the experimental results of a microhole array and a phase step show that the proposed method has better adaptability and a higher precision. Moreover, the applicability of this method for different uses is also demonstrated by experiments with a microhole array and a phase step. © 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.OE.54.3.031103] Keywords: digital holography; spatial filtering; region growing. Paper 140931SS received Jun. 10, 2014; revised manuscript received Aug. 3, 2014; accepted for publication Aug. 7, 2014; published online Oct. 14, 2014. 1 Introduction Digital holography is widely used in the fields of microelectromechanical systems measurement,1–6 biological cell monitoring, 7–12 microscopic particles tracking,13,14 and ladar observation systems15,16 due to its significant advantages over other conventional three-dimensional (3-D) measurements such as high resolution,17,18 real time,8,9,13,14,19 and nonmechanical scanning.20–22 In order to get a good quality reconstructed image, the zero order of diffraction and the real image must be eliminated during digital reconstruction.23–25 Phase-shifting digital holography,26,27 off-axis digital holography, 25,28,29 and parallel phase-shifting digital holography30,31 are commonly used to eliminate the two undesired diffraction images. The first one is not suitable for real-time dynamic analysis due to the multiple holograms required. The latter one is fit for real-time dynamic analysis but its spatial resolution is low. However, off-axis digital holography is not only fit for real-time dynamic analysis, but also has high spatial resolution. So, off-axis digital holography is widely employed in real-time dynamic analysis. However, proper spatial filtering is necessary to determine the þ1 term spectrum which will affect the quality of the reconstructed image. To realize real-time analysis, it is very important to automatically and precisely find out the distribution of the þ1 term spectrum. There are two methods of spatial filtering used to achieve the þ1 term spectrum, including manual spatial filtering17,32 and automatic spatial filtering.33,34 Manual spatial filtering is time consuming and depends on subjective judgment. Therefore, it is not suitable for real time and dynamic analysis. However, in typical automatic spatial filtering, a key step is to set a regular filtering window (an elliptic filtering window or a rectangular filtering window) for eliminating the zero and −1 term spectra (representing the zero order of diffraction and the