| 论文标题 |
Specific detection of carcinoembryonic antigen based on fluorescencequenching of Au-Ag core-shell nanotriangle probe |
| 作者 |
Jian Zhu∗, Jing-Fei Wang, Jian-Jun Li, Jun-Wu Zhao∗ |
| 发表/完成日期 |
2016-10-05 |
| 期刊名称 |
Sensors and Actuators B: Chemical |
| 期卷 |
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| 相关文章 |
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| 论文简介 |
Specific detection of carcinoembryonic antigen based on fluorescencequenching of Au-Ag core-shell nanotriangle probe
Gold-silver bimetallic fluorescence quenching nanoprobe has been synthesized based on the gold triangu-lar nanoplate with silver coating. In order to guarantee the specificity of the probe, surface modificationsof the triangular nanoplates including carboxyl replacement, carboxyl activation and antibody connec-tion have been carried out. The fluorescence spectral study indicates that the fluorescence intensityof carcinoembryonic antigen (CEA) could be effectively quenched by the silver coated-gold triangularnanoprobe. And the fluorescence quenching ability could be improved by optimizing the interactiontime and amount of binding anti-CEA to modified nanoprobe, and the interaction time of binding CEAto anti-CEA. By using this sensing method, the CEA could be detected with an ultra low detection limitof 10 pg/mL, and the linear range spans a scope from 20 to 200 pg/mL. Interference experiment resultsshow that the influence from the interfering proteins could be neglected in the detection procedure. Andthe CEA could be specifically detected by using this bimetallic triangular nanoplate probe.© 2016 Elsevier B.V. All rights reserved. |
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