主要论文 - 杨 伯伦
论文标题 | Hydrolysis of O-nitrophenyl-beta-D-galactoside by an enzyme immobilized on perfluorocarbon in a tapered column reactor |
作者 | Yang BL, Shinoda N, Goto S |
发表/完成日期 | 1998-03-15 |
期刊名称 | COLLOIDS AND SURFACES B-BIOINTERFACES |
期卷 | 10 (3) |
相关文章 | |
论文简介 | An enzyme, beta-galactosidase, was immobilized on the surface of perfluorocarbon (PFC) droplets using perfluorobenzyl chloride as a modifier. The optimal conditions for introducing the enzyme to the PFC were determined in order to find the maximal activity for the hydrolysis of O-nitrophenyl-beta-D-galactoside. This novel immobilized enzyme was also successfully used in a tapered column reactor. Experimental results indicated that PFC droplets tended to stabilize the operation in a tapered column reactor with upflow and allowed a much wider range of operating conditions than a conventional cylindrical column reactor. (C) 1998 Elsevier Science B.V. IDS Number: ZD192 |