Daocheng Wu was born in
Currently he’s research is focused on the areas of biomaterials, bionanotechnology, and related topics that include drug delivery system, nanoparticles for diagnosis and therapy, as well as functional nanoparticles and their applications in the biomedical field. In recent 10 years, he has published over 120 papers in many peer-review journals. He has published more than 70 research papers in advanced functional materials (IF 15.7), biomaterials (IF 10.3), J material Chem a (IF10.4), nanoscale (IF7.3), autophagy (IF11.3), ACS appl. Material. Inter (IF8.1) and other famous SCI journals. His cittation is more than 7500 times in total and H factor 24. Among them, there are more than 60 articles written by the first and corresponding authors. He has published more than 100 articles in core journals in China. More than 30 doctoral and master degree students have been trained.
1.Antitumor effects of gossypol and its derivatives
A lot of studies have been carried out on the separation and purification of gossypol and its derivatives as well as the anti-cancer research of its derivatives. For the first time, gossypol derivatives have been found to have the mechanism of autophagy of tumor cells, and the synergistic effect of gossypol derivatives and adriamycin on tumors has been found. They are assembled into mulberry-like double-drug targeting self-assembly. In the assembled nano-drug carrier system, only 1/5 dosage of the drug can achieve 1.5 times the original therapeutic effect, and the toxicity and side effects are greatly reduced. The synergistic effect of gossypol derivatives with other drugs was also found. More than 20 papers hace been published in Adv Funct Mater, Biomaterials, Cancer Biology and Treatment Cancer, J Mater Chem B, J Control Release and other international well-known journals ,
2. Ultrasound Drug Controlled Release System and Equipment
A gelatin ionized nanogel with reversible ultrasound triggered intelligent drug release was designed and prepared. The response time and recovery time of the gelatin ionized nanogel were 1-2 min and 3-4 min， respectively. The average particle size of the gelatin ionized nanogel was increased to 2-3 micron after reversible ultrasound triggered, which greatly increased the drug load of the system. A high photothermal conversion efficiency system of polydopamine and near infrared dye IR-780 was designed to modify perfluorocarbon nanodroplets with sensitive photothermal phase transition ability. Under the action of near infrared laser, the temperature of the system has been greatly increased from room temperature to above 60 C, which is enough to realize the hyperthermia of tumor cells and tissues. In addition, perfluorocarbon nanodroplets undergo obvious phase transition (from liquid to gaseous). The perfluorocarbon gas after phase transition can enhance the contrast effect of ultrasound, visualize the tumor site of photothermal therapy, and the intensity of ultrasound signal also indicates the temperature change process and degree of hyperthermia. In this study, perfluorocarbon nanodroplets were used to achieve in vivo treatment of animals and real-time monitoring of treatment status. More than 10 papers have been published in ACS Applied Materials & Interfaces, Current Medicinal Chemistry, Nanoscale, J Mater Chem B, C and other well-known international journals. A number of invention patents have been also granted.
3 Nanothermometer for biomedical applications
A new method for the formation of fluorescent nanothermometer by encapsulating water-soluble rhodamine F-127 fluorescent dye and Rhodamine B (reference) in melamine nanoparticles at the same time was studied. The temperature sensitivity of the fluorescent nanothermometer is high (7.6%C-1). It can measure temperature in a wide temperature range from -20 C to 150 C. It has been successfully applied to microwave heating of cells measurement. The fluorescence resonance energy transfer (FRET) of random curl size Rg of single stranded DNA molecule was used to measure the microenvironment temperature. A colorimetric fluorescent nanometric thermometer based on single stranded DNA has been developed. It has high sensitivity (7.04% degree C-1) and wide temperature range (0-100 degrees C). It has a stable thermo sensitive property and successfully applied to the quantitative measurement of gel and intracellular temperature. More than 10 papers have been published in ACS Applied Materials & Interfaces, J Mater Chem B, C and other well-known international journals.
9.Multi-functional core-shell immunomagnetic bead for the research of cancer stem cells.
10 . Extract process of Gossypol acetic acid from cottonseed soapstock and (-) Gossypol separation
(Shaanxi province science and technology innovation project , 2011KTCL03-07)
18.YonghuaZhan ,GuangfengJia , Daocheng Wu*, YiqingXu ,and LiangXu. Designand Synthesis of a Gossypol Derivative with Improved AntitumorActivities. Arch.Pharm.Chem.Life Sci.2009,342,223–229.
20.Daocheng Wu* , Mingxi Wan, Hong Wu . Ultrasonic triggered release drug delivery system of Pluronic micelle. Nanoscience, 2006,11(3):210-218
21.Xian-Qing Zhang, Xiao-Feng Huang, Shi-Jie Mu, Qun-Xing An, Ai-Jun Xia, Rui Chen, Dao-Cheng Wu* Inhibition of proliferation of prostate cancer cell line, PC-3,in vitro and in vivo using (–)-gossypol. Asian Journal of Andrology 2010,12:390-399.
22.Wu D*, Wan M. Preparation of the core-shell structure adriamycin lipiodol microemulsions and their synergistic anti-tumor effects with diethyldithiocarbamate in vivo. Biomedicine & Pharmacotherapy 2010, 64(9):615-23.
23.Xian-Qing Zhang, Xiao-Feng Huang, Xing-Bin Hu, Yong-Hua Zhan, Qun-Xing An, Shi-Ming Yang, Ai- Jun Xia,Jing Yi, Rui Chen, Shi-Jie Mu, Dao-Cheng Wu*. Apogossypolone, a novel inhibitor of antiapoptotic Bcl-2 family proteins, induces autophagy of PC-3 and LNCaP prostate cancer cells in vitro. Asian Journal of Andrology. 2010;12:697-708.
24.Xinghua Pan, Jianhui Ju, Yonghua Zhan, and Daocheng Wu*. Preparation and Fluorescence Characteristics of Amido- functionalized Dual-fluorescent Microspheres with Core-shell Structure Micromolecular Chemistry & Physics . 2010, 221 (21) ;2347-2355 .
25.Xinghua Pan , Rachel Thompson , Xiaojie Meng , Daocheng Wu , Liang Xu. Tumor-targeted RNA-interference: functional non-viral nanovectors. Am J Cancer Res 2011;1(1):32-49(invited Review)
26.Xinghua Pan, Jianhui Ju, Jianjun Li and Daocheng Wu*. Poly(styrene-acrylamide- acrylic acid) copolymer fluorescent microspheres with improved hydrophilicity: preparation and influence on protein immobilization. High Performance Polymers 2011，22（3）；255-262
27.Liu T, Wu D. Simultaneous determination of some ultraviolet-absorbing chemicals in sunscreen cosmetics using a high-performance liquid chromatography method. Int J Cosmet Sci. 2011, 33, 408–415.
28.Zhenhai Gan, Jianhui Ju, Ting Zhang, and Daocheng Wu*. Preparation of Rhodamine B Fluorescent Poly(methacrylic acid) Coated Gelatin Nanoparticles. J Nanomaterials 2011, Article ID 753705, 8 pages doi:10.1155/2011/753705.
29.Wei Gao, Baoyong Sha, Wei Zou, Xuan Liang, Xiangzhong Meng, Hao Xu, Jun Tang, Daocheng Wu,* Lixian Xu, Hui Zhang. Cationic Amylose-Encapsulated Bovine Hemoglobin as a Nanosized Oxygen Carrier. Biomaterials 2011,32:9425-9433.
30.Chen L, Kong X, Liang Z, Ye F, Yu K, Dai W, Wu D*, Luo C*, Jiang H. Theoretical Study of the Mechanism of Proton Transfer in the Esterase Estb from Burkholderia Gladioli. Journal of Physical Chemistry B. 2011, 115 (44):13019–13025.
31.Lu XY, Wu DC, Li ZJ, Chen GQ. Polymer nanoparticles. Prog Mol Biol Transl Sci. 2011;104:299-323.
32. Ruifen Xu, Xuyang Feng, Xin Xie, Jin Zhang, Daocheng Wu*, Lixian Xu*. HIV-1 Tat protein increase the permeability of brain endothelial cells by both inhibiting Occludin expression and cleaving occludin via matrix metalloproteinase-9. Brain Research 2012,1436:13-19.
33.Wu Daocheng*, Wan Mingxi. Methylene diphosphonate-conjugated adriamycin liposomes: Preparation, characteristics, and targeted therapy for osteosarcomas in vitro and in vivo. Biomedical Microdevices 2012,14:497-510
43.Youshen Wu, Yan Li, Jianhua Xu and Daocheng Wu*.Incorporating Fluorescent Dyes into Monodisperse
Melamine-Formaldehyde Resin Microspheres via Organic Sol-Gel Process: Pre-polymer Doping Strategy.J. Mater. Chem. B, 2014, 2（35），5837-5846 .
44.Hao Liu, Ke Li, Lan Lan, Jingwen Ma, Yun Zeng, Liang Xu*and Daocheng Wu*.Double-layered hyaluronic acid/stearic acidmodified polyethyleneimine nanoparticles encapsulating (-)-gossypol: a nanocarrier for chiral anticancer drugs.J. Mater. Chem. B, 2014 2,5238-5248.45.Ke Li , Hao Liu , Wei Gao , Mu Chen, Yun Zeng , Jiajun Liu, Liang Xu ,Daocheng Wu*,Mulberry-like dual-drug complicated nanocarriers assembled with apogossypolone amphiphilic starch micelles and doxorubicin hyaluronic acid nanoparticles for tumor combination and targeted therapy. Biomaterials 2015,39,131-144
based on low molecular weight PEI for tumor-targeted in vivo imaging. Journal Nanopart Research 2014, 16: 2784
delivery system based on gas-filled BSA microbubbles and gelatin nanogels. Journal of Controlled release 2015,213;e24 (doi:10.1016/j.jconrel.2015.05.036)
53.Yiao Yu, Youshen Wu , Jiajun Liu, Yonghua Zhan , Daocheng Wu* Ultrasmall dopamine-coated nanogolds: preparation, characteristics, and CT imaging Journal of Experimental Nanoscience 2016,11(Sup1):S1-S11 (DOI:10.1080/ 17458080. 2015.1102343)
54.Yao Yu, Youshen Wu, Jiajun Liu, Ke Li, Daocheng Wu* Metabolizable dopamine-coated gold nanoparticles aggregates: Preparation, characteristics, computed tomography imaging, acute toxicity, and metabolism in vivo Journal of Materials Chemistry B, 2016, 4,1090-1099(DOI: 10.1039/C5TB02497H )
55. Zhang X, Xi X, Li M , Wu D*. Comparison of Impulse Radar and Spread-Spectrum Radar in Through-Wall Imaging IEEE Transactions on Microwave Theory and Techniques , 2016 ,64(3):699-706(DOI:10.1109/TMTT.2015.2508791).
56.Hao Liu and Daocheng Wu*. In vivo near-infrared fluorescence tumor imaging using DiR-loaded nanocarriers.Current Drug Delivery 2016,13(1):40-48 (DOI: 10.2174/1567201812666150703114908. )
57. Yun Zeng, Jingwen Ma, Shaojuan Zhang, and Daocheng Wu,* Imaging Agents in Targeting Tumor Hypoxia. Current Medicinal Chemistry, 2016(17), 23, 1775-1800.
58..Yongxing Dua,Lingze Zhang,Lulu Sangeand Daocheng Wu,* Temperature field simulation and phantom validation of a Two-armed Spiral Antenna for microwave thermotherapy.Technology and Health Care 2016,24 S675–S682.
59.Youshen Wu, Jiajun Liu, Jingwen Ma, Yongchun Liu, Ya Wang, and Daocheng Wu*.Ratiometric Nanothermometer Based on Rhodamine Dye-Incorporated F127-Melamine-Formaldehyde Polymer Nanoparticle:Preparation, Characterization, Wide-Range Temperature Sensing,and Precise Intracellular Thermometry.ACS Appl. Mater. Inter 2016,8(23):14396-14405.
60.Wei Gao , Yongchun Liu , Guixia Jing , Ke Li , Yuan Zhao , Baoyong Sha Qiang Wang *,Daocheng Wu *. Rapid and efficient crossing blood-brain barrier: Hydrophobic drugdelivery system based on propionylated amylose helix nanoclusters.Biomaterials 2017;113 :133-144 (DOI : 10.1016/ j.biomaterials.2016.10.045
61.Jingwen Ma, Yun Zeng, Yongchun Liu and Daocheng Wu*.Thermostable polymeric nanomicelles of iridium(III) complexes with aggregation-induced phosphorescence emission characteristics and their recyclable double-strand DNA monitoring. J. Mater. Chem B. 2017,5,123-133.
62. Youshen Wu,Liu Jiajun, Ya Wang, Ke Li , Jianhua Xu , Daocheng Wu*. Novel ratiomatric flourescence nanothermometers based on flouropores-labeled short single-stranded DNA. ACS Appl. Mater. Inter 2017,9(12):11073-11081.
63. Shihong Shen,Youshen Wu, Yongchun LIu, Daocheng Wu*. High drug-loading nanomedicine:progress, current statues, and prospects. Int J Nanomedicine 2017,12;4085-4109 ( invited Review)
64.Yongchun Liu ,Chunhong Zhang ,Xizhe LI, Daocheng Wu*. A self-monitored fluorescence .DNA anti-counterfeiting system based on silica coated SYBR Green I/DNA gelatin nanoparticles. J Mater, Chem C , 2017, 5,5939-3948 (DOI: 10.1039/C7TC01380A ) (This article is part of the themed collection 2017 Journal of Materials Chemistry C HOT Papers)
65.Jiajun Liu, Youshen Wu, Yao Yu, Ke Li, Yuanyuan Ji, Daocheng Wu *. Quantitative ratiometric phosphorescence hypoxia–sensing nanoprobes based on Quantum Dots/Ir(III) glycerol monoolein cubic-phase nanoparticles.
66.Yao Yu, Youshen Wu, Yongchun Liu ,,Liu Jiajunm, Daocheng Wu*. Highly efficient dual-modal phosphorescence/ computed tomography bioprobes based on iridium complex and AuNPs polyiohexol composite nanoparticles ,Nanoscale, 2017, 9,9447-9456
67. Xiaoying lei, Ke li, Yan liu, Zhen Yu Wang, Ban Jun Ruan, Li Wang, An Xiang, Daocheng Wu*, Zifan lu*. Co-delivery nanocarriers targeting folate receptor and encapsulating 2-deoxyglucose and a-tocopheryl succinate enhance anti-tumor effect in vivo. International Journal of Nanomedicine 2017,12,5701-5715.
68. Lu Lei, Peng Tang, Yongchun Liu, Ke Li, Jiajun Liu, Daocheng Wu*. Controlled thermo-triggered drug release system based on propionylated amylose helix nanoclusters. Journal of Controlled Release ,2017,259 ;e89–e90
69. Yongchun Liu,Wei Gao,Chunghong Zhang,Peng Tang, Yuan Zhao, Daocheng Wu*.Sequenential molecule-triggered-release system based on acetylated amylose helix aggregates. Chem Comm 2017,53,10680-10683.
70. Shihong Shen ,Youshen Wu ,Ke Li, Ya Wang Jimin Wu Yun Zeng, Daocheng Wu*. Versatile hyaluornic acid modified AQ4N-Cu(II)-gossypol infinite coordination polymer nanoparticles:Mulitiple tumor targeting,highle efficient synergistic chemotherapy and real-time self-montoring.Biomaterials 2018,154:197-212
72.Ying Wang , Ya Wang , Ji Wang , Wan Lei , Ke Li , Daocheng Wu∗ , Xiaojuan Wang∗ Pharmacokinetics, biodistribution, and bioavailability of gossypol-loaded Pluronic ® F127 nanoparticles Journal of Drug Delivery Science and Technology 2018，45；388-396
73. Ailiang Zhang*, Xumei Yang, Quanxiao Li, Yu Wang, Gan Zhao, Bin Wang, Daocheng Wu*. Immunostimulatory activity of wat er-extractable polysaccharides from Cistanchedeserticola as a plant adjuvant in vitro and in vivo. PlosOne 2018,13;1:e0191356
74. Peng Tang , Yongchun Liu , Yiming Liu , Hongru Meng , Zeying Liu , Ke Li , Daocheng Wu* Thermochromism-induced temperature self-regulation and alternating photothermal nanohelix clusters for synergistic tumor chemo/photothermal therapy. Biomaterials 2019,88;12-23
75 Ya Wang, Youshen Wu,Ke Li , Shihong Shen, Zeying Liu, Daocheng Wu*. Ultralong Circulating Lollipop‐Like Nanoparticles Assembled with Gossypol, Doxorubicin, and Polydopamine via π–π Stacking for Synergistic Tumor Therapy. Advanced Functional Materials 2019,29(1);1805582 doi.org/10.1002/adfm.201805582
77. Hui Zhang,Youshen Wu,Zhenhai Gan,,Yuexuan Yang,Yiming Liu,,Peng Tang and Daocheng Wu*.Accurate intracellular and in vivo temperature sensing based on CuInS2/ZnS QD micelles.J. Mater. Chem B. 2019,7,2835--2844.
78.Hongrui Zhu, Zhenhai Gan, Dapeng Li, Jing Qin, Hongmei Zhang, Mingxi Wan, Daocheng Wu *. Sensitive detection of dopamine with ultrasound cavitation-enhanced fluorescence method. Microchemical Journal 2019, 150; 104199.
79.Yiming Liu, Ke Li, Youshen Wu, Jingwen Ma, Peng Tang, Yongchun Liua and Daocheng Wu*. PVA reinforced gossypolone and doxorubicin π–π stacking nanoparticles towards tumor targeting and ultralow dose synergistic chemotherapy. Biomaterials Science 2019,7. 3662-2674.
80 Hao Liu, Rebecca T. Marquez, Xiaoqing Wu, Ke Li, Shweta Vadlamani, Song Li, Ya Wang, Liang Xu * and Daocheng Wu *.A non-intrusive evaluation method for tumortargeting characteristics of nanomedicines based on in vivo near-infrared fluorescence imaging.J. Mater. Chem B. 2019,7, 4751- 4757.
81 Yun Zeng, Jingwen Ma, Liang Xu *, Daocheng Wu *. Natural Product Gossypol and Its Derivatives in Precision Cancer. Current Medicinal Chemistry. 2019,26; 1849-1873 ( DOI: 10.2174/ 09298673246661 70523123655 )
82.Lingze Zhang, Zeying Liu, Yiming Liu, Ya Wang, Peng Tang, Youshen Wu, Hengbo Huang, Zhenhai Gan, Jiajun Liu, Daocheng Wu∗ Ultrathin surface coated water-soluble cobalt ferrite nanoparticles with high magnetic heating eﬃciency and rapid in vivo clearance 、Biomaterials 2020，230:119655
83.Yang Yuexuan & Wu Daocheng* ,Research shortcomings of fluorescent nanothermometers in biological and medical fields, Nanomedicine(Lond.) (Epub ahead of print) 2020 DOI10.2217/nnm-2019-0416
84 Zeying Liu, Lingze Zhang, Peng Xiaoa, Lingfeng Liu, Peng Tang, Ya Wang, Shihong Shen,Jiajun Liu, Zhenhai Gan, Daocheng Wu*.Magnetothermally responsive composite submicron particles for recyclable catalytic applications.Chemical Engineering Journal 2020,399:125554
85.Weiqiu Jin, Changzi Dong, Dengtian Yang, Ruotong Zhang, Tianshu Jiang and Daocheng Wu* .Nano-Carriers of Combination Tumor Physical Stimuli-Responsive Therapies .Current Drug Delivery, 2020, 17 （11）；577-587 (DOI: 10.2174/1567201817666200525004225 )
86. Ya Wang, Siyuan Luo, Youshen Wu, Peng Tang, Jiajun Liu, Zeying Liu, Shihong Shen, Haozhe Ren, and Daocheng Wu*. Highly Penetrable and On-Demand Oxygen Release with Tumor Activity Composite Nanosystem for Photothermal/Photodynamic Synergetic Therapy. ACS nano 2020 ，14；17046-17062( https://dx.doi.org/10.1021/acsnano.0c06415)