张剑峰
- 研究员
- 博士生导师
- 硕士生导师
- 教师英文名称：Jianfeng Zhang
- 电子邮箱：
- 所在单位：基础医学院
- 学历：博士研究生毕业
- 办公地点：西安交通大学创新港校区和雁塔校区
- 性别：男
- 联系方式：
- 学位：博士学位
- 毕业院校：上海交通大学
- 学科：微生物学,病原生物学
- 学科：微生物学
病原生物学
The pivotal role of IncFIB(Mar) plasmid in the emergence and spread of hypervirulent carbapenem-resistant Klebsiella pneumoniae strains
- 发布时间：2025-01-31
- DOI码：10.1126/sciadv.ado9097
- 论文名称：The pivotal role of IncFIB(Mar) plasmid in the emergence and spread of hypervirulent carbapenem-resistant Klebsiella pneumoniae strains
- 发表刊物：Science Advances
- 摘要：The hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) poses a substantial challenge to the global health care. However, the mechanism behind its evolution and transmission remain elusive. Here, four virulence plasmid types were identified from 310 hv-CRKP isolates collected nationwide during 2017–2018, based on their aerobactin (iuc locus) lineage and IncFIB replicons. Notably, pIUC1-IncFIB(K)37 and pIUC1-IncFIB(Mar), representing two epidemic virulence plasmids in Asia and Europe, respectively, accounted for >90% of the hv-CRKP episodes. Analysis of 494 K. pneumoniae isolates (376 from 2010–2013; 118 from 2017–2018) and 2578 public K. pneumoniae genomes indicated the notable role of IncFIB(Mar) plasmids in the hv-CRKP emergence and spread. Conjugation assays showed the helper IncFIB(Mar) plasmid could efficiently transfer into a hypervirulent strain and uniquely retromobilize with pIUC1-IncFIB(K)37 back into CRKP. Thereafter, the IncFIB(Mar) plasmid either lost rapidly or recombined with pIUC1-IncFIB(K)37, generating the hybrid pIUC1-IncFIB(Mar) plasmid. Our
findings elucidated formation, evolution, and dissemination trajectories of the two major hv-CRKP strains in different regions. - 是否译文：否