张剑峰
- 研究员
- 博士生导师
- 硕士生导师
- 教师英文名称：Jianfeng Zhang
- 电子邮箱：
- 所在单位：基础医学院
- 学历：博士研究生毕业
- 办公地点：西安交通大学创新港校区和雁塔校区
- 性别：男
- 联系方式：
- 学位：博士学位
- 毕业院校：上海交通大学
- 学科：微生物学,病原生物学
- 学科：微生物学
病原生物学
Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor
- 发布时间：2024-08-09
- DOI码：10.1093/nar/gkae674
- 论文名称：Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor
- 发表刊物：Nucleic Acids Research
- 摘要：Tn3 family transposons are a widespread group of replicative transposons, notorious for contributing to the dissemination of antibiotic resistance, particularly the global prevalence of carbapenem resistance. The transposase (TnpA) of these elements catalyzes DNA breakage and rejoining reactions required for transposition. However, the molecular mechanism for target site selection with these elements remains unclear. Here, we identify a QLxxLR motif in N-terminal of Tn3 TnpAs and demonstrate that this motif allows interaction between TnpA of Tn3 family transposon Tn1721 and the host β-sliding clamp (DnaN), the major processivity factor of the DNA replication machinery. The TnpA-DnaN interaction is essential for Tn1721 transposition. Our work unveils a mechanism whereby Tn3 family transposons can bias transposition into certain replisomes through an interaction with the host replication machinery. This study further expands the diversity of mobile elements that use interaction with the host replication machinery to bias integration.
- 是否译文：否